Rat CD56/NCAM-1 ELISA Kit PicoKine®

Mfr.No.

EK1562

Sensitivity

<20 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.004	0.064	0.118	0.215	0.450	0.798	1.578	2.017

Description

Rat CD56/NCAM-1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Ncam1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Neural cell adhesion molecule 1;N-CAM-1;NCAM-1;CD56;Ncam1;Ncam;

Molecular Weight

94658 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: L20-T712

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat CD56

Subcellular Localization

Cell membrane; Single-pass type I membrane protein.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	1208	4356	19046	1152	4200	19885
Standard deviation	57.98	204.73	1047.53	72.57	201.6	1411.83
CV (%)	4.8%	4.7%	5.5%	4.8%%	4.8%	7.1%

Protein Function

This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.

Background

Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cellcell adhesion, neurite outgrowth, synaptic plasticity, and leaing and memory. This gene encodes a cell adhesion protein which is a member of the immunoglobulin superfamily. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. And the encoded protein has been shown to be involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	1208	1266	1216	1295	1246	35.86	2.8%
Sample 2	4356	3976	3792	4024	4037	203.51	5%
Sample 3	19046	21680	19420	19020	19791	1101.75	5.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24586
Gene Name	Ncam1
UniProt ID	P13596
Protein Name	Neural cell adhesion molecule 1

Applications	ELISA
Research Area	Axonal Guidance Proteins,Cell Adhesion Proteins,Cell Type Marker,Developmental Biology,ECM Proteins,Growth and Development,Hematopoietic Progenitors,Membrane Proteins,Nervous System Development,Neural Signal Transduction,Neurology Process,Neuron Marker,Neuroscience,Organogenesis,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001136	EK1562	96 wells/kit	$499		Add to Cart

Rat CD56/NCAM-1 ELISA Kit PicoKine®

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