Matrices listed below were spiked with certain level of CD40L and the recovery rates were calculated by comparing the measured value to the expected amount of CD40L in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-93
92
EDTA plasma(n=5)
85-98
91
heparin plasma(n=5)
87-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CD40L and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
CD40L ELISA Kit allows for the in vitro quantitative determination of CD40L concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit