Rat CD36/SR-B3/GP4 ELISA Kit PicoKine®

Mfr.No.

EK1299

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.072	0.182	0.319	0.435	0.689	1.087	1.539	2.037

Description

Rat CD36/SR-B3 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cd36 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Platelet glycoprotein 4;Adipocyte membrane protein;Fatty acid translocase;Fatty acid transport protein;Glycoprotein IIIb;GPIIIB;PAS IV;PAS-4;Platelet glycoprotein IV;GPIV;CD36;Cd36;Fat;

Molecular Weight

52731 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: G30-K439

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat CD36

Subcellular Localization

Cell membrane; Multi-pass membrane protein. Upon ligand-binding, intealized through dynamin-dependent endocytosis. .

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	297	1163	5833	317	1160	5856
Standard deviation	16.92	91.87	425.8	19.65	92.8	462.62
CV (%)	5.7%	7.9%	7.3%	6.2%	8%	7.9%

Protein Function

Binds to collagen, thrombospondin, anionic phospholipids and oxidized low-density lipoprotein (oxLDL). May function as a cell adhesion molecule. ly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes. Binds long chain fatty acids and may function in the transport and/or as a regulator of fatty acid transport (By similarity). Receptor for thombospondins, THBS1 AND THBS2, mediating their antiangiogenic effects (By similarity). As a coreceptor for TLR4-TLR6, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42 binding, rapidly induces the formation of a heterodimer of TLR4 and TLR6, which is intealized and triggers inflammatory signals, leading to the NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion. .

Background

CD36 (Cluster of Differentiation 36) is an integral membrane protein found on the surface of many cell types in vertebrate animals and is also known as FAT, SCARB3, GP88, glycoprotein IV (gpIV) and glycoprotein IIIb (gpIIIb). The human CD36 is a member of a gene family of structurally related glycoproteins and functions as a receptor for collagen type I and thrombospondin. The use of a CD36 genomic probe has allowed the localization of the CD36 locus to the 7q11.2 band by fluorescence in situ hybridization coupled with GTG-banding. CED-1/SCARF1 and C03F11.3/CD36 are beta-glucan binding receptors and define an evolutionarily conserved pathway for the innate sensing of fungal pathogens.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	297	267	314	299	294	17.04	5.7%
Sample 2	1163	1212	1289	1172	1209	49.73	4.1%
Sample 3	5833	5270	6084	5144	5582	388.64	6.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	29184
Gene Name	CD36
UniProt ID	Q07969
Protein Name	Platelet glycoprotein 4
Tissue Specificity	Expressed at high levels in heart, intestine, spleen, adipose tissue, skeletal muscle and, at lower levels, in testes. .

Applications	ELISA
Research Area	Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Cell Type Markers,Defensin,Heart Disease,Hematopoietic Progenitors,Human Protein,Immunology,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathway,Metabolic Signaling Pathways,Metabolism,Metabolism of Lipids and Lipoproteins,Microbiology,Pathways and Processes,Platelets,Protein,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000892	EK1299	96 wells/kit	$499		Add to Cart

Rat CD36/SR-B3/GP4 ELISA Kit PicoKine®

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