Rat CD25/IL2RA ELISA Kit PicoKine®

Mfr.No.

EK2271

Sensitivity

<45 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration(pg/ml)	0	93.7	187.5	375	750	1,500	3,000	6,000
O.D.	0.019	0.084	0.156	0.395	0.581	0.881	1.275	1.831

Description

Rat CD25/IL2RA ELISA Kit PicoKine® (96 Tests). Quantitate Rat CD25/IL2RA in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 45 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: CHO; Immunogen sequence: E22-Q235

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat Il2ra

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	114	306	2233	155	316	2793
Standard deviation	6.68	15.67	64.09	10.37	22.03	161.99
CV(%)	5.9%	5.1%	2.9%	6.7%	7%	5.8%

Background

CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	114	154	167	139	144	4.69	3.3%
Sample 2	306	243	257	203	252	19.55	7.8%
Sample 3	2233	2818	2456	2488	2499	151.67	6.1%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	25704
Gene Name	Il2ra
UniProt ID	P26897

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001820	EK2271	96 wells/kit	$499		Add to Cart

Rat CD25/IL2RA ELISA Kit PicoKine®

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