Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of CBb and the recovery rates were calculated by comparing the measured value to the expected amount of CBb in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-97
92
EDTA plasma(n=5)
89-104
98
heparin plasma(n=5)
85-99
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of CBb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-104%
86-101%
89-100%
EDTA plasma(n=5)
86-98%
86-100%
86-101%
heparin plasma(n=5)
86-97%
86-99%
86-100%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
CBbELISA Kit allows for the in vitro quantitative determination of CBb concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat CBb (Complement Factor Bb) ELISA Kit