Rat Cathepsin K - CATH-K ELISA Kit

Mfr.No.

E0790Ra

Sensitivity

0.016ng/ml

Detection Range

0.03-15ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Cathepsin K (also known as cath-K) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cathepsin K;CTSK

Molecular Weight

36791

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0790Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.9	0.11	5.5
Intra-Assay	2	18	3.1	0.16	5.3
Intra-Assay	3	18	0.5	0.02	4.2

Background

Thiol protease involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. Involved in the release of thyroid hormone thyroxine (T4) by limited proteolysis of TG/thyroglobulin in the thyroid follicle lumen.

Protocol

These standard curves of E0790Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.069	2.146	2.105
	2.223
4ng/ml	1.938	1.815	1.774
	1.691
2ng/ml	1.306	1.246	1.206
	1.186
1ng/ml	0.664	0.707	0.667
	0.75
0.5ng/ml	0.35	0.398	0.357
	0.446
0ng/ml	0.038	0.04	0
	0.042

Gene ID	29175
UniProt ID	O35186

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112042	E0790Ra	48T	$320		Add to Cart
BHE12112042	E0790Ra	96T	$458		Add to Cart

Rat Cathepsin K - CATH-K ELISA Kit

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