Rat Cathepsin D - CATH-D ELISA Kit

Mfr.No.

E1180Ra

Sensitivity

0.12ng/ml

Detection Range

0.3-60ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Cathepsin D (also known as Cath-D) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

cath-D;Cathepsin D;Cathepsin D 12 kDa light chain;Cathepsin D 30 kDa heavy chain);Cathepsin D 34 kDa heavy chain;Cathepsin D 9 kDa light chain

Molecular Weight

44681

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1180Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	27.62	0.97	3.52
Intra-Assay	2	18	14.56	0.87	5.95
Intra-Assay	3	18	6.7	0.4	5.94

Background

Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation.

Protocol

These standard curves of E1180Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.418	2.401	2.349
	2.383
20ng/ml	1.369	1.386	1.335
	1.403
10ng/ml	0.689	0.785	0.734
	0.881
5ng/ml	0.54	0.494	0.443
	0.448
2.5ng/ml	0.264	0.253	0.202
	0.243
0ng/ml	0.054	0.051	0
	0.049

Gene ID	171293
UniProt ID	P24268

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112427	E1180Ra	48T	$320		Add to Cart
BHE12112427	E1180Ra	96T	$458		Add to Cart

Rat Cathepsin D - CATH-D ELISA Kit

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