Rat Catechol-O-methyltransferase

Mfr.No.

E1319Ra

Sensitivity

9.49ng/L

Detection Range

20-4500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Catechol-O-Methyltransferase (also known as COMT) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Catechol O-methyltransferase;COMT

Molecular Weight

29597

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1319Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1567.58	46.63	2.97
Intra-Assay	2	18	942.74	46.53	4.94
Intra-Assay	3	18	276.93	17.96	6.48

Background

Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol.

Protocol

These standard curves of E1319Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.332	2.328	2.269
	2.323
1200ng/L	1.295	1.307	1.248
	1.319
600ng/L	0.702	0.701	0.641
	0.699
300ng/L	0.405	0.48	0.42
	0.554
150ng/L	0.271	0.272	0.213
	0.273
0ng/L	0.049	0.059	0
	0.07

Gene ID	24267
UniProt ID	P22734

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112558	E1319Ra	48T	$320		Add to Cart
BHE12112558	E1319Ra	96T	$458		Add to Cart

Rat Catechol-O-methyltransferase - COMT ELISA Kit

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