Rat Caspase-1(CASP1) ELISA kit

Rat Caspase-1(CASP1) ELISA kit

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Mfr.No.

CSB-EL004543RA

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase), ICE, IL1BC, P45, CASP1 nirs variant 1, IL1B-convertase, caspase 1, caspase 1, apoptosis-related cysteine protease (interleukin 1, beta, convertase), caspase-1, interleukin 1-B converting enzyme, interleuk

Recovery

The recovery of canine PA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	94-105
EDTA plasma (n=4)	89	84-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine PA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	95
	Range %	91-98
1:200	Average %	103
	Range %	99-106
1:400	Average %	98
	Range %	93-104
1:800	Average %	92
	Range %	87-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

?g/ml	OD1	OD2	Average
10	0.145	0.139	0.142
5	0.275	0.262	0.269
2.5	0.512	0.550	0.531
1.25	0.913	0.983	0.948
0.625	1.665	1.560	1.613
0	2.647	2.432	2.540

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P43527

Applications	ELISA
Research Area	CASP1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10507727	CSB-EL004543RA	96T	$695		Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.