Rat Cardiac Troponin T(cTn-T)ELISA kit

Rat Cardiac Troponin T(cTn-T)ELISA kit

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Mfr.No.

CSB-E16443r

Sensitivity

3.12 pg/mL

Detection Range

12.5 pg/mL-800 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Cardiac Troponin T(cTn-T)

Recovery

The recovery of human HMW adiponectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-104
EDTA plasma (n=4)	92	87-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HMW adiponectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	87
	Range %	84-91
1:200	Average %	95
	Range %	90-100
1:400	Average %	99
	Range %	95-104
1:800	Average %	101
	Range %	96-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.169	2.117	2.143	2.040
100	1.679	1.658	1.669	1.566
50	1.245	1.231	1.238	1.135
25	0.832	0.869	0.851	0.748
12.5	0.513	0.547	0.530	0.427
6.25	0.345	0.367	0.356	0.253
3.12	0.203	0.212	0.208	0.105
0	0.102	0.104	0.103	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P50753

Applications	ELISA
Research Area	cTn-T

Storage	Store at 2-8°C. Please refer to protocol.

Reference	THE POSSIBLE CARDIOPROTECTIVE EFFECTS OF DIFFERENT FRACTIONS OF ARTICHOKE EXTRACTS AGAINST 5-FU INDUCED CARDIOTOXICITY IN ALBINO RATS Najim S M.et al,International Journal of Pharmacy and Pharmaceutical Sciences,7(11): 165-169. Roselle Polyphenols Exert Potent Negative Inotropic Effects via Modulation of Intracellular Calcium Regulatory Channels in Isolated Rat Heart. Lim YC.et al,Cardiovasc Toxicol.,Jan 9 Alleviation of Cardiac Damage by Dietary Fenugreek (Trigonella foenum-graecum) Seeds is Potentiated by Onion (Allium cepa) in Experimental Diabetic Rats via Pradeep SR.et al,Cardiovasc Toxicol,Nov 1 Effect of betaine supplement on isoprenaline induced myocardial infarction and serum cathepsin G level in rat model Soroush Ghodratizadeh .et al,J Urmia Univ Med Sci ,Nov 22 Inhibition of nuclear factor kappa B pathway protects myocardial ischemia/reperfusion injury in rats under treatment with abnormal savda munziq Maimaitiaili, Abudunaibi.et al,Am J Transl Res,/ The effect of eight weeks aerobic exercise on troponin T and metallothionein levels of cardiac tissue in healthy male rats Saeedeh Shadmehri.et al,/,January 6-Gingerol Protects Heart by Suppressing Myocardial Ischemia/Reperfusion Induced Inflammation via the PI3K/Akt-Dependent Mechanism in Rats Tongtong Xu, et al,Evidence-Based Complementary and Alternative Medicine,November 5 A novel hypertensive crisis rat model established by excessive norepinephrine infusion and the potential therapeutic effects of Rho-kinase inhibitors on it Tian-YiYuan, et al,Biomedicine & pharmacotherapy,November 26

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.