Rat Carboxypeptidase B(CPB1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL005883RA

Sensitivity

3.12 ng/mL

Detection Range

12.5 ng/mL-800 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

carboxypeptidase B1 (tissue), CPB, DKFZp779K1333, PASP, PCPB, pancreas-specific protein, pancreatic carboxypeptidase B1, procarboxypeptidase B

Recovery

The recovery of human L-Carnitine spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	82-110
EDTA plasma (n=4)	97	90-110

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human L-Carnitine in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:200	Average %	88
	Range %	80-96
1:400	Average %	97
	Range %	91-105
1:800	Average %	99
	Range %	92-110
1:1600	Average %	100
	Range %	90-110

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














ng/ml	OD1	OD2	Average	Corrected
20	2.749	2.639	2.694	2.543
10	2.010	1.976	1.993	1.842
5	1.303	1.294	1.299	1.148
2.5	0.889	0.839	0.864	0.713
1.25	0.561	0.524	0.543	0.392
0.625	0.371	0.359	0.365	0.214
0.312	0.256	0.239	0.248	0.097
0	0.155	0.147	0.151	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P19223

Applications	ELISA
Research Area	CPB1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507714	CSB-EL005883RA	96T	$695	Add to Cart
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BHE10507714	CSB-EL005883RA	10x96T	$6602.50	Add to Cart