Rat Calprotectin (CALP) ELISA kit

Mfr.No.

CSB-EQ013485RA

Sensitivity

15.6 ng/mL

Detection Range

62.5 ng/mL-4000 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Calprotectin

Recovery

The recovery of rabbit F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	110	107-113

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:20	Average %	85
	Range %	80-90
1:40	Average %	104
	Range %	100-107
1:80	Average %	87
	Range %	83-90
1:160	Average %	102
	Range %	98-106

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	CALP

Storage	Store at 2-8°C. Please refer to protocol.

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