Rat Calpain-1 Catalytic Subunit

Mfr.No.

E1324Ra

Sensitivity

0.028ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Calpain-1 Catalytic Subunit (also known as CAPN1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Calcium-activated neutral proteinase 1;Calpain mu-type;Calpain-1 catalytic subunit;Calpain-1 large subunit;CANP 1;CAPN 1;CAPN1;Micromolar-calpain;muCANP;

Molecular Weight

82119

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1324Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.8	0.43	6.4
Intra-Assay	2	18	14.3	0.52	3.6
Intra-Assay	3	18	3.8	0.24	6.2

Background

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-364', 'Gly-377' and 'His-399'.

Protocol

These standard curves of E1324Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.351	2.222	2.181
	2.093
10ng/ml	1.398	1.281	1.24
	1.164
5ng/ml	0.64	0.731	0.69
	0.823
2.5ng/ml	0.428	0.475	0.434
	0.521
1.25ng/ml	0.227	0.203	0.162
	0.179
0ng/ml	0.032	0.041	0
	0.051

Gene ID	29153
UniProt ID	P97571

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112563	E1324Ra	48T	$320		Add to Cart
BHE12112563	E1324Ra	96T	$458		Add to Cart

Rat Calpain-1 Catalytic Subunit - CAPN1 ELISA Kit

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