Rat Calpain 1 - CAPN1 ELISA Kit

Mfr.No.

E1748Ra

Sensitivity

0.049ng/ml

Detection Range

0.1-40ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Calcium-activated neutral proteinase 1;Calpain mu-type;Calpain-1 catalytic subunit;Calpain-1 large subunit;CANP 1;CAPN 1;CAPN1;Micromolar-calpain;muCANP;

Molecular Weight

82119

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1748Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	24.8	1.03	4.1
Intra-Assay	2	18	12.4	0.48	3.9
Intra-Assay	3	18	6.1	0.27	4.5

Background

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-364', 'Gly-377' and 'His-399'.

Protocol

These standard curves of E1748Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/ml	1.753	1.764	1.739
	1.776
12ng/ml	0.952	0.991	0.965
	1.03
6ng/ml	0.499	0.489	0.463
	0.479
3ng/ml	0.177	0.186	0.161
	0.195
1.5ng/ml	0.121	0.134	0.108
	0.146
0ng/ml	0.026	0.026	0
	0.026

Gene ID	29153
UniProt ID	P97571

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112954	E1748Ra	48T	$320		Add to Cart
BHE12112954	E1748Ra	96T	$458		Add to Cart

Rat Calpain 1 - CAPN1 ELISA Kit

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