Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Calcrl and the recovery rates were calculated by comparing the measured value to the expected amount of Calcrl in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-98
94
EDTA plasma(n=5)
93-99
96
heparin plasma(n=5)
91-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcrl and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-105%
91-103%
88-97%
EDTA plasma(n=5)
92-99%
90-99%
89-101%
heparin plasma(n=5)
94-98%
95-96%
94-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q63118
Applications
ELISA
Applications Notes
Calcrl ELISA Kit allows for the in vitro quantitative determination of Calcrl concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Calcrl (Calcitonin gene-related peptide type 1 receptor) ELISA Kit