Rat calcitonin gene related peptide,CGRP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E08211r

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

calcitonin gene related peptide,CGRP

Recovery

The recovery of human SAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-97
EDTA plasma (n=4)	96	92-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	96
	Range %	92-101
1:4	Average %	93
	Range %	85-95
1:8	Average %	100
	Range %	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P10093

Applications	ELISA
Research Area	CGRP

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Granulocyte colony-stimulating factor provides protection against cardiovascular autonomic neuropathy in streptozotocin-induced diabetes in rats Erba.et al,Diabetes Res Clin Pract.,2015 107(3):377-83. Calcitonin gene-related peptide in aerobic exercise induces collateral circulation development in rat ischemia myocardium YuanHui Wang.et al,Biomedicine & Pharmacotherapy,82,561-567 Bone adaptation to mechanical loading in a mouse model of reduced peripheral sensory nerve function Heffner MA.et al,PLoS One,Oct 31

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