Rat Calcitonin,CT ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E05132r

Alternative Names

calcitonin, CALCA Calcitonin

Reactivity

Rat

Detection Range

1.56 pg/mL-100 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Duration

1-5h

Recovery

The recovery of human AP12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	103-110
EDTA plasma (n=4)	89	85-92

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human AP12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-95
1:2	Average %	87
	Range %	84-90
1:4	Average %	84
	Range %	81-87
1:8	Average %	93
	Range %	90-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

0.39 pg/mL

Limit Of Detection

450 nm

UniProt ID	P01257
Antigen	calcitonin-related polypeptide alpha

Applications

ELISA

Research Area

CALCA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Metallothionein deficiency aggravates depleted uranium-induced nephrotoxicity Hao Y. et al,Toxicol Appl Pharmacol. ,2015 Sep 15;287(3):306-15.

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