Matrices listed below were spiked with certain level of Calcb and the recovery rates were calculated by comparing the measured value to the expected amount of Calcb in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
92-103
97
EDTA plasma(n=5)
85-102
95
heparin plasma(n=5)
85-97
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Calcb and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Calcb ELISA Kit allows for the in vitro quantitative determination of Calcb concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Calcb(Calcitonin gene-related peptide 2) ELISA Kit