Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of C4a and the recovery rates were calculated by comparing the measured value to the expected amount of C4a in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
93-104
100
EDTA plasma(n=5)
89-99
92
heparin plasma(n=5)
88-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of C4a and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
C4a ELISA Kit allows for the in vitro quantitative determination of C4a concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat C4a (Complement Component 4a) ELISA Kit