Rat brain natriuretic peptide,BNP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07972r

Alternative Names

brain natriuretic peptide,BNP, BNP, brain type natriuretic peptide, natriuretic protein

Reactivity

Rat

Detection Range

125 pg/mL-8000 pg/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human ANX-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-96
EDTA plasma (n=4)	95	89-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ANX-?in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	92-101
1:2	Average %	90
	Range %	82-96
1:4	Average %	101
	Range %	99-104
1:8	Average %	87
	Range %	82-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

31.25 pg/mL

Limit Of Detection

450 nm

UniProt ID	P13205
Antigen	brain natriuretic peptide,BNP

Applications

ELISA

Research Area

BNP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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