Rat Bone Morphogenetic Protein 2

Mfr.No.

E0276Ra

Sensitivity

2.51ng/L

Detection Range

5-1500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BMP 2;BMP2;BMP-2;BMP-2A;Bone morphogenetic protein 2;Bone morphogenetic protein 2A

Molecular Weight

44383

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0276Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	699.4	15.62	2.2
Intra-Assay	2	18	199.2	9.49	4.8
Intra-Assay	3	18	338.7	19.46	5.7

Background

Induces cartilage and bone formation. Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4.

Protocol

These standard curves of E0276Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	1.854	1.856	1.817
	1.857
400ng/L	0.887	0.883	0.844
	0.879
200ng/L	0.571	0.596	0.557
	0.62
100ng/L	0.24	0.236	0.197
	0.233
50ng/L	0.079	0.107	0.068
	0.135
0ng/L	0.028	0.039	0
	0.05

Gene ID	29373
UniProt ID	P49001

Research Area	Cardiovascular; Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111577	E0276Ra	48T	$320		Add to Cart
BHE12111577	E0276Ra	96T	$458		Add to Cart

Rat Bone Morphogenetic Protein 2 - BMP-2 ELISA Kit

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