Rat bone alkaline phosphatase,BALP ELISA Kit

Mfr.No.

CSB-E11865r

Alternative Names

bone alkaline phosphatase,BALP

Reactivity

Rat

Detection Range

15.6 U/L-1000 U/L

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human FGF18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	102	96-108
EDTA plasma (n=4)	96	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FGF18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

Range %

89-107

1:2

Average %

Range %

87-96

1:4

Average %

Range %

90-100

1:8

Average %

Range %

94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.303	2.227	2.265	2.113
1000	1.685	1.765	1.725	1.573
500	1.182	1.163	1.173	1.021
250	0.809	0.815	0.812	0.660
125	0.466	0.454	0.460	0.308
62.5	0.344	0.334	0.339	0.187
31.25	0.220	0.228	0.224	0.072
0	0.152	0.151	0.152	?

Species

Rattus norvegicus

Sensitivity

3.9 U/L

Limit Of Detection

450 nm

UniProt ID	P08289
Antigen	bone alkaline phosphatase,BALP

Applications

ELISA

Research Area

BALP

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Characterization of Salvia Miltiorrhiza ethanol extract as an anti-osteoporotic agent /,BMC Complementary and Alternative Medicine,2011 Nov 28;11:120. Dose-dependent effects of genistein on bone homeostasis in rats' mandibular subchondral bone /,Acta Pharmacologica Sinica,2012 Jan;33(1):66-74. Advanced glycation end products suppress lysyl oxidase and induce bone collagen degradation in a rat model of renal osteodystrophy Aoki C et al,Lab Invest,2013 Aug 26. Experimental development of bisphosphonate-related osteonecrosis of the jaws in rodents. Conte Neto N et al,Int J Exp Pathol,2013 Feb;94(1):65-73. Effects of Chronic Stress and Alendronate Therapy on the Osseointegration of Titanium Implants Conte Neto N et al,Clin Implant Dent Relat Res.,2013 Feb 28. Icariin Protects Against Glucocorticoid-Induced Osteoporosis In Vitro and Prevents Glucocorticoid-Induced Osteocyte Apoptosis In Vivo Feng R et al,Cell Biochem Biophys,2013 Feb 17. Advanced Oxidation Protein Products Accelerate Bone Deterioration in Aged Rats Zeng JH et al,Exp Gerontol,2013 Dec 4.pii: S0531-5565(13)00343-4. Evaluation of the effects of gastrectomy on the development of metabolic bone disease Yuki Higashi.et al,Journal of Surgical Research,Dec 12 Effects of curcumin and melatonin on bone formation in orthopedically expanded suture in rats: A biochemical, histological and immunohistochemical study M. G. Cesur.et al,ORTHOD CRANIOFAC RES,June 21 Bone health consequence of adjuvant anastrozole in monotherapy or associated with biochanin-A in ovariectomized rat model Mohamed AA.et al,Life Sciences,Octobert 2

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Rat bone alkaline phosphatase,BALP ELISA Kit

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