Rat BMP-9 ELISA Kit PicoKine®

Mfr.No.

EK1611

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.053	0.121	0.149	0.209	0.385	0.749	1.406	2.096

Description

Rat BMP-9 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Gdf2 in bone tissue, cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Growth differentiation factor 2 ;Similar to Growth/differentiation factor 2 (GDF-2) (Bone morphogenetic protein 9) (BMP-9) ;Gdf2 ;LOC290921 ;rCG_42312 ;

Molecular Weight

47978 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: S320-R430

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat BMP-9

Subcellular Localization

Secreted .

Sample Type

Bone tissue homogenate, Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	36	187	414	35	192	435
Standard deviation	2.12	11.78	23.59	2.69	13.63	27.16
CV (%)	5.7%	7.1%	5.7%	7.7%	7.1%	5.9%

Background

Growth differentiation factor 2 (GDF2) also known as bone morphogenetic protein (BMP)-9 is a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates cartilage and bone development, angiogenesis and differentiation of cholinergic central nervous system neurons. Homozygous null mice exhibit malformations of the vasculature and skeleton.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	36	36	36	38	36	0.86	2.3%
Sample 2	187	182	199	172	185	9.72	5.2%
Sample 3	414	464	457	446	445	19.14	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	290921
Gene Name	Gdf2
UniProt ID	M0RB87

Applications	ELISA
Research Area	Cell Biology,Cell Cycle,Cell Differentiation,Cytoskeleton/ECM,Extracellular Matrix,Growth Factors/Hormones,Signal Transduction,Structures

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001283	EK1611	96 wells/kit	$499		Add to Cart

Rat BMP-9 ELISA Kit PicoKine®

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