Rat BMP-7 ELISA Kit PicoKine®

Mfr.No.

EK1444

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.038	0.114	0.176	0.282	0.504	0.900	1.583	2.255

Description

Rat BMP-7 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Bmp7 in bone tissue, cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bone morphogenetic protein 7 ;Protein Bmp7 ;Bmp7 ;rCG_40866 ;

Molecular Weight

49199 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: S292-H430

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat BMP-7

Subcellular Localization

Secreted .

Sample Type

Bone tissue homogenate, Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	86	190	752	93	186	766
Standard deviation	4.38	14.44	36.09	5.76	16.55	49.79
CV (%)	6.5%	7.6%	4.8%	6.2%	8.9%	6.5%

Background

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-? superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	86	94	87	84	87	3.76	4.3%
Sample 2	190	232	197	213	208	16.17	7.7%
Sample 3	752	848	801	765	791	37.23	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	85272
Gene Name	Bmp7
UniProt ID	G3V6W8
Protein Name	Bone morphogenetic protein 7

Applications	ELISA
Research Area	Cancer,Cell Biology,Cell Cycle,Cell Differentiation,Cytoskeleton/ECM,Developmental Biology,Extracellular Matrix,Growth Factors/Hormones,Lipid and Lipoprotein Metabolism,Lipid Metabolism,Metabolic Signaling Pathways,Metabolism,Nervous System Development,Organogenesis,Pathways and Processes,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,Structures,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001053	EK1444	96 wells/kit	$499		Add to Cart

Rat BMP-7 ELISA Kit PicoKine®

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