Rat BMP-2 ELISA Kit PicoKine®

Mfr.No.

EK0312

Sensitivity

<2 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.002	0.026	0.060	0.114	0.349	0.608	1.152	1.989

Description

Rat BMP-2 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Bmp2 in bone tissue, cell culture supeatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Bone morphogenetic protein 2;BMP-2;Bone morphogenetic protein 2A;BMP-2A;Bmp2;Bmp-2;

Molecular Weight

44383 MW

Immunogen

Expression system for standard: CHO; Immunogen sequence: Q283-R396

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat BMP-2

Subcellular Localization

Secreted .

Sample Type

Bone tissue homogenate, Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	96	245	806	105	234	777
Standard deviation	6.72	11.27	49.16	9.24	14.97	52.83
CV (%)	7%	4.6%	6.1%	8.8%	6.4%	6.8%

Protein Function

Induces cartilage and bone formation.

Background

Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	96	96	106	100	99	4.09	4.1%
Sample 2	245	258	242	225	242	11.75	4.8%
Sample 3	806	902	918	836	865	46.09	5.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	29373
Gene Name	BMP2
UniProt ID	P49001
Protein Name	Bone morphogenetic protein 2
Tissue Specificity	Expressed in femur, calvaria, trachea, lung and ovary.

Applications	ELISA
Research Area	Angiogenesis,Cancer,Cardiovascular,Cytoskeleton/ECM,Extracellular Matrix,Growth Factors,Growth Factors/Hormones,Neural Stem Cells,Secreted,Signal Transduction,Signaling Pathways,Stem Cells,Structures,TGF Beta

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000349	EK0312	96 wells/kit	$499		Add to Cart

Rat BMP-2 ELISA Kit PicoKine®

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