Rat Biglycan - BGN ELISA Kit

Mfr.No.

E1888Ra

Sensitivity

0.043ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

BGN;Biglycan;Bone/cartilage proteoglycan I;BSPG1;PG-S1

Molecular Weight

41706

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1888Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	10.7	0.52	4.88
Intra-Assay	2	18	16.07	0.36	2.24
Intra-Assay	3	18	5.95	0.28	4.77

Background

Biglycan is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. In humans, biglycan is encoded by the BGN gene which is located on the X chromosome.

Protocol

These standard curves of E1888Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.027	2.013	1.97
	2
12ng/mL	1.037	1.017	0.973
	0.997
6ng/mL	0.49	0.481	0.437
	0.472
3ng/mL	0.207	0.253	0.209
	0.298
1.5ng/mL	0.095	0.125	0.081
	0.155
0ng/mL	0.053	0.044	0
	0.034

Gene ID	25181
UniProt ID	P47853

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113087	E1888Ra	48T	$320		Add to Cart
BHE12113087	E1888Ra	96T	$458		Add to Cart

Rat Biglycan - BGN ELISA Kit

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