Matrices listed below were spiked with certain level of beta Arrestin-2 and the recovery rates were calculated by comparing the measured value to the expected amount of beta Arrestin-2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
95
EDTA plasma(n=5)
88-101
94
heparin plasma(n=5)
92-104
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of beta Arrestin-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-104%
87-94%
90-105%
EDTA plasma(n=5)
90-100%
90-101%
83-93%
heparin plasma(n=5)
80-100%
81-96%
87-92%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
P29067
Applications
ELISA
Applications Notes
beta Arrestin-2 ELISA kit allows for the in vitro quantitative determination of beta Arrestin-2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat beta Arrestin-2 (Beta Arrestin-2) ELISA kit