Matrices listed below were spiked with certain level of beta Arrestin-1 and the recovery rates were calculated by comparing the measured value to the expected amount of beta Arrestin-1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-100
93
EDTA plasma(n=5)
85-102
94
heparin plasma(n=5)
93-100
98
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of beta Arrestin-1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-105%
87-105%
87-97%
EDTA plasma(n=5)
87-100%
87-100%
83-95%
heparin plasma(n=5)
82-95%
88-96%
83-98%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
P29066
Applications
ELISA
Applications Notes
beta Arrestin-1 ELISA kit allows for the in vitro quantitative determination of beta Arrestin-1 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat beta Arrestin-1 (Beta Arrestin- 1) ELISA kit