Rat Beclin-1(BECN1) ELISA kit

Mfr.No.

CSB-EL002658RA

Alternative Names

beclin 1, autophagy related, ATG6, VPS30, beclin1, ATG6 autophagy related 6 homolog, beclin 1, beclin 1 (coiled-coil, moesin-like BCL2 interacting protein), beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)

Reactivity

Rat

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	98	90-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse PDGF-CC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	101
	Range %	95-105
1:2	Average %	95
	Range %	89-100
1:4	Average %	96
	Range %	88-101
1:8	Average %	94
	Range %	90-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
4000	2.480	2.411	2.446	2.282
2000	1.879	1.858	1.869	1.705
1000	1.151	1.109	1.130	0.966
500	0.636	0.625	0.631	0.467
250	0.399	0.389	0.394	0.230
125	0.287	0.278	0.283	0.119
62.5	0.215	0.210	0.213	0.049
0	0.166	0.162	0.164	?

Species

Rattus norvegicus

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q91XJ1
Antigen	beclin 1, autophagy related

Applications

ELISA

Research Area

BECN1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10507661	CSB-EL002658RA	96T	$695	Add to Cart
BHE10507661	CSB-EL002658RA	5x96T	$3336	Add to Cart
BHE10507661	CSB-EL002658RA	10x96T	$6602.50	Add to Cart

Rat Beclin-1(BECN1) ELISA kit

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