Rat Bckdk([3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial) ELISA Kit

Mfr.No.

ER6010

Alternative Names

Bckdk ELISA Kit, (3-methyl-2-oxobutanoate dehydrogenase (lipoamide)) kinase ELISA Kit, mitochondrial ELISA Kit, Branched-chain alpha-ketoacid dehydrogenase kinase ELISA Kit, BCKD-kinase ELISA Kit, BCKDHKIN ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

78.125-5000pg/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Bckdk and the recovery rates were calculated by comparing the measured value to the expected amount of Bckdk in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	90-101	93
EDTA plasma(n=5)	87-103	96
heparin plasma(n=5)	89-105	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Bckdk and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	92-103%	89-99%	90-104%
EDTA plasma(n=5)	83-98%	84-99%	83-97%
heparin plasma(n=5)	85-89%	80-100%	80-98%

Sensitivity

< 46.875pg/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Notes

For Research Use Only

UniProt ID	Q00972
Antigen	BCKDK

Applications	ELISA
Applications Notes	Bckdk ELISA Kit allows for the in vitro quantitative determination of Bckdk concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10804514	ER6010	96T	$450		Add to Cart

Rat Bckdk ([3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase, mitochondrial) ELISA Kit

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