Matrices listed below were spiked with certain level of BCHE and the recovery rates were calculated by comparing the measured value to the expected amount of BCHE in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-102
96
EDTA plasma(n=5)
85-99
93
heparin plasma(n=5)
87-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCHE and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-105%
90-104%
86-98%
EDTA plasma(n=5)
87-100%
86-99%
86-100%
heparin plasma(n=5)
91-99%
86-97%
87-97%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
BCHE ELISA Kit allows for the in vitro quantitative determination of BCHE concentrations in serum, plasma, tissue homogenates and other biological fluids.