Rat BAD (BCL-2 Associated Death Promoter) CLIA Kit

Mfr.No.

E-CL-R0071

Sensitivity

7.5 pg/mL

Detection Range

12.5-800 pg/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Description

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat BAD. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BAD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAD, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat BAD. You can calculate the concentration of Rat BAD in the samples by comparing the RLU value of the samples to the standard curve.

Detection Method

Chemiluminescence

Recovery

80%-120%

Linearity

Samples were spiked with high concentrations of Rat BAD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

		Serum (n=5)	EDTA plasma (n=5)	Cell culture media (n=5)
1:2	Range (%)	101-116	85-100	97-110
1:2	Average (%)	108	91	105
1:4	Range (%)	95-111	89-105	101-116
1:4	Average (%)	102	96	110
1:8	Range (%)	95-110	88-104	90-101
1:8	Average (%)	101	95	96
1:16	Range (%)	95-106	85-99	89-101
1:16	Average (%)	100	90	96

Specificity

This kit recognizes Rat BAD in samples.No significant cross-reactivity or interference between Rat BAD and analogues was observed

Components

An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	8 wells ×12 strips	-20℃, 6 months
Reference Standard	2 vials
Concentrated Biotinylated Detection Ab (100×)	1 vial, 120 μL
Concentrated HRP Conjugate (100×)	1 vial, 120 μL	-20℃(shading light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	4℃, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	4℃ (shading light)
Substrate Reagent B	1 vial, 5 mL	4℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Precision

Both intra-CV and inter-CV are < 10%.

Background

This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ANG1 Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ANG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain

Usage Notes

This ELISA kit applies to the in vitro quantitative determination of Rat BAD concentrations in Serum, plasma and other biological fluids.

UniProt ID	O35147

Applications	CLIA
Research Area	Cancer, Cell Biology, Metabolism

Storage	2-8°C/-20°C,6 months
Shipping	Ice bag

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SKU	Supplier No.	Size	Your price	Quantity
BHE10301947	E-CL-R0071	96T	$682		Add to Cart
BHE10301947	E-CL-R0071	48T	$546		Add to Cart

Rat BAD (BCL-2 Associated Death Promoter) CLIA Kit

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