Rat B7-1/CD80 ELISA Kit PicoKine®

Mfr.No.

EK0709

Sensitivity

<2 pg/ml

Detection Range

62.5 pg/ml - 4,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.019	0.06	0.092	0.178	0.406	0.799	1.611	2.283

Description

Rat B7-1/CD80 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Cd80 in cell culture supeatants and serum. Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

B7.1 ;Cd80 ;B7.1 ;

Molecular Weight

36352 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: I39-Q248

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat CD80

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	154	448	1666	165	472	1688
Standard deviation	10.78	21.5	89.96	13.69	29.26	113.09
CV (%)	7%	4.8%	5.4%	8.3%	6.2%	6.7%

Background

The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7-1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain.The CD80 and CD86 genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types. B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	154	156	159	178	161	9.54	5.9%
Sample 2	448	401	380	439	417	27.7	6.6%
Sample 3	1666	1734	1536	1629	1641	71.48	4.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	25408
Gene Name	CD80
UniProt ID	O35187
Protein Name	T-lymphocyte activation antigen CD80

Applications	ELISA
Research Area	Adaptive Immunity,B Cells,Hematopoietic Progenitors,Immunology,Lymphoid,Myeloid,Stem Cells

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000581	EK0709	96 wells/kit	$499		Add to Cart

Rat B7-1/CD80 ELISA Kit PicoKine®

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