Matrices listed below were spiked with certain level of AZGP and the recovery rates were calculated by comparing the measured value to the expected amount of AZGP in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
98
EDTA plasma(n=5)
89-104
98
heparin plasma(n=5)
89-103
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AZGP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-98%
85-100%
87-97%
EDTA plasma(n=5)
87-97%
86-97%
82-98%
heparin plasma(n=5)
83-90%
81-99%
90-99%
Sensitivity
< 1.875ng/ml
Notes
For Research Use Only
UniProt ID
Q63678
Applications
ELISA
Applications Notes
AZGP1 ELISA Kit allows for the in vitro quantitative determination of AZGP concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat AZGP1(Zinc-alpha-2-glycoprotein) ELISA Kit