Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Atp5b and the recovery rates were calculated by comparing the measured value to the expected amount of Atp5b in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-103
93
EDTA plasma(n=5)
97-102
100
heparin plasma(n=5)
87-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Atp5b and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Atp5b ELISA Kit allows for the in vitro quantitative determination of Atp5b concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Atp5b (ATP synthase subunit beta, mitochondrial) ELISA Kit