Matrices listed below were spiked with certain level of Atp1a3 and the recovery rates were calculated by comparing the measured value to the expected amount of Atp1a3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-105
95
EDTA plasma(n=5)
87-100
91
heparin plasma(n=5)
89-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Atp1a3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Atp1a3 ELISA Kit allows for the in vitro quantitative determination of Atp1a3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Atp1a3(Sodium/potassium-transporting ATPase subunit alpha-3) ELISA Kit