Rat Atp-binding Cassette Sub-family G Member 8

Mfr.No.

E1247Ra

Sensitivity

10.21ng/L

Detection Range

20-6000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat ATP-Binding Cassette Sub-Family G Member 8 (also known as ABCG8) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

ABCG8;ATP-binding cassette sub-family G member 8;Sterolin-2

Molecular Weight

78236

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1247Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	513.86	21.54	4.19
Intra-Assay	2	18	1601.83	74.08	4.62
Intra-Assay	3	18	2828.2	120.69	4.27

Background

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg2+- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of the dietary cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG5 has ATPase activity.

Protocol

These standard curves of E1247Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4000ng/L	2.126	2.182	2.147
	2.238
2000ng/L	1.515	1.412	1.378
	1.31
1000ng/L	0.653	0.642	0.608
	0.631
500ng/L	0.33	0.359	0.325
	0.389
250ng/L	0.189	0.199	0.164
	0.208
0ng/L	0.049	0.034	0
	0.02

Gene ID	155192
UniProt ID	P58428

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112490	E1247Ra	48T	$320		Add to Cart
BHE12112490	E1247Ra	96T	$458		Add to Cart

Rat Atp-binding Cassette Sub-family G Member 8 - ABCG8 ELISA Kit

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