Rat Atp-binding Cassette Sub-family G Member 5

Mfr.No.

E1246Ra

Sensitivity

7.21ng/L

Detection Range

15-3000ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat ATP-Binding Cassette Sub-Family G Member 5 (also known as ABCG5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ABCG 5;ABCG5;ATP-binding cassette sub-family G member 5;Sterolin-1

Molecular Weight

73372

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1246Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	816.3	28.33	3.5
Intra-Assay	2	18	540.7	29.26	5.4
Intra-Assay	3	18	234.3	16.58	7.1

Background

ABCG5 and ABCG8 form an obligate heterodimer that mediates Mg(2+)- and ATP-dependent sterol transport across the cell membrane. Plays an essential role in the selective transport of dietary plant sterols and cholesterol in and out of the enterocytes and in the selective sterol excretion by the liver into bile. Required for normal sterol homeostasis. The heterodimer with ABCG8 has ATPase activity.

Protocol

These standard curves of E1246Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.439	2.444	2.382
	2.449
800ng/L	1.223	1.216	1.153
	1.208
400ng/L	0.859	0.87	0.807
	0.88
200ng/L	0.548	0.498	0.436
	0.449
100ng/L	0.297	0.272	0.21
	0.248
0ng/L	0.05	0.062	0
	0.074

Gene ID	114628
UniProt ID	Q99PE7

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112489	E1246Ra	48T	$320		Add to Cart
BHE12112489	E1246Ra	96T	$458		Add to Cart

Rat Atp-binding Cassette Sub-family G Member 5 - ABCG5 ELISA Kit

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