Matrices listed below were spiked with certain level of ATG12 and the recovery rates were calculated by comparing the measured value to the expected amount of ATG12 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-105
95
EDTA plasma(n=5)
85-98
92
heparin plasma(n=5)
85-99
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ATG12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-105%
87-103%
86-104%
EDTA plasma(n=5)
84-95%
86-99%
82-100%
heparin plasma(n=5)
91-100%
82-95%
86-100%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q2TBJ5
Applications
ELISA
Applications Notes
ATG12 ELISA Kit allows for the in vitro quantitative determination of ATG12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat ATG12 ( autophagy protein 12 ) ELISA Kit