Rat asymmetrical dimethylarginine(ADMA) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13039r

Sensitivity

0.75 ng/mL

Detection Range

1.5 ng/mL-300 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

asymmetrical dimethylarginine(ADMA)

Recovery

The recovery of human FOXO3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FOXO3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	87
	Range %	80-92
1:200	Average %	99
	Range %	91-105
1:400	Average %	101
	Range %	92-110
1:800	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	ADMA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Regulation of endothelial nitric oxide synthase and asymmetric dimethylarginine by matrine attenuates isoproterenol-induced acute myocardial injury in rats /,Journal of pharmacy and pharmacology,Aug;64(8):1107-18. Comparative Effect Mutlu E.et al,Clinical & Experimental Pharmacology,/ Protective Effects of Low Dose Rosuvastatin on Isoproterenol-induced Chronic Heart Failure in Rats by Regulation of DDAH-ADMA-NO Pathway Ru Zhou.et al,Cardiovascular Therapeutics,Dec 13

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