Matrices listed below were spiked with certain level of Asrgl1 and the recovery rates were calculated by comparing the measured value to the expected amount of Asrgl1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-101
94
EDTA plasma(n=5)
88-101
95
heparin plasma(n=5)
91-102
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Asrgl1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Asrgl1 ELISA Kit allows for the in vitro quantitative determination of Asrgl1 concentrations in serum, plasma, tissue homogenates and other biological fluids.