Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Asprosin and the recovery rates were calculated by comparing the measured value to the expected amount of Asprosin in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-103
94
EDTA plasma(n=5)
94-104
100
heparin plasma(n=5)
85-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Asprosin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-102%
93-102%
88-104%
EDTA plasma(n=5)
82-99%
87-101%
82-97%
heparin plasma(n=5)
92-99%
80-94%
92-97%
Sensitivity
< 37.5pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Asprosin ELISA Kit allows for the in vitro quantitative determination of Asprosin concentrations in serum, plasma, tissue homogenates and other biological fluids.