Rat Arachidonic Acid(AA) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13008r

Sensitivity

2.758 ng/mL

Detection Range

3.125 ng/mL-200 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Arachidonic Acid(AA)

Recovery

The recovery of Human YWHAQ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	96-108
EDTA plasma (n=4)	88	82-94

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human YWHAQ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	93
	Range %	90-97
1:4	Average %	85
	Range %	81-89
1:8	Average %	98
	Range %	94-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	AA

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Advanced Glycation End Products (AGEs) level and insulin resistance in women with gestational diabetes Zainab NE. Aziz B.Sc. et al,/,2015 3(4):416-423. Protective effect of low dose gadolinium chloride against isoproterenol-induced myocardial injury in rat Zheng YY. et al,Apoptosis.,2015 Sep;20(9):1164-75 Pretreatment with low-dose gadolinium chloride attenuates myocardial ischemia/reperfusion injury in rats Chen M.et al,Acta Pharmacol Sin.,2016 37(4):453-62. Molecular mechanism of Danshensu on platelet antiaggregation Chen Yu.et al,American Institute of Physics,April

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