Rat Arachidonate 5-lipoxygenase (Alox5) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E16982r

Sensitivity

0.039 ng/mL

Detection Range

0.156 ng/mL-10 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Arachidonate 5-lipoxygenase (Alox5), RP11-67C2.3, 5-LO, 5-LOX, 5LPG, LOG5, MGC163204, arachidonic acid 5-lipoxygenase, leukotriene A4 synthase

Recovery

The recovery of mouse FBLN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-97
EDTA plasma (n=4)	94	90-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse FBLN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1000	Average %	95
	Range %	89-99
1:2000	Average %	95
	Range %	91-101
1:4000	Average %	94
	Range %	89-98
1:8000	Average %	98
	Range %	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P12527

Applications	ELISA
Research Area	Alox5

Storage	Store at 2-8°C. Please refer to protocol.

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