Matrices listed below were spiked with certain level of AR and the recovery rates were calculated by comparing the measured value to the expected amount of AR in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-99
95
EDTA plasma(n=5)
86-104
96
heparin plasma(n=5)
87-98
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AR and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.