Rat apolipoprotein B100 (Apo-B100) ELISA Kit

Mfr.No.

CSB-E08102r

Sensitivity

0.342 ug/mL

Detection Range

0.313 ug/mL-20 ug/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

apolipoprotein B (including Ag(x) antigen), FLDB, LDLCQ4, apoB-100, apoB-48, apolipoprotein B, apolipoprotein B48

Recovery

The recovery of horse HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-98
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of horse HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-95
1:2	Average %	102
	Range %	97-107
1:4	Average %	91
	Range %	85-97
1:8	Average %	97
	Range %	91-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	Q7TMA5

Applications	ELISA
Research Area	APOB

Storage	Store at 2-8°C. Please refer to protocol.

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