Rat Apolipoprotein B - APO-B ELISA Kit

Mfr.No.

E1356Ra

Sensitivity

0.02mmol/L

Detection Range

0.05 -3mmol/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Apolipoprotein B (also known as Apo-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Apo B-100;Apo B-48;APOB;Apolipoprotein B-100;Apolipoprotein B-48

Molecular Weight

536024

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1356Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	0.6	0.04	5.6
Intra-Assay	2	18	1	0.03	3.3
Intra-Assay	3	18	0.3	0.02	6.3

Background

Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.

Protocol

These standard curves of E1356Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1.6mmol/L	2.42	2.357	2.299
	2.295
0.8mmol/L	1.346	1.377	1.319
	1.409
0.4mmol/L	0.813	0.794	0.736
	0.776
0.2mmol/L	0.43	0.431	0.373
	0.431
0.1mmol/L	0.261	0.272	0.213
	0.282
0mmol/L	0.07	0.058	0
	0.046

Gene ID	54225
UniProt ID	Q7TMA5

Research Area	Cardiovascular; Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112592	E1356Ra	48T	$320		Add to Cart
BHE12112592	E1356Ra	96T	$458		Add to Cart

Rat Apolipoprotein B - APO-B ELISA Kit

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