Rat Apolipoprotein A1 - APO-A1 ELISA Kit

Mfr.No.

E0743Ra

Sensitivity

0.022ug/mL

Detection Range

0.05-20ug/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Apoprotein A1 (also known as APO-A1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

APOA 1;APOA1;ApoA-I;Apo-AI;Apolipoprotein A1;Apolipoprotein A-I;ProapoA-I;Proapolipoprotein A-I

Molecular Weight

30062

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0743Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.9	0.24	3.5
Intra-Assay	2	18	5.5	0.25	4.6
Intra-Assay	3	18	3.7	0.18	4.7

Background

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.

Protocol

These standard curves of E0743Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ug/mL	2.023	2.032	2.003
	2.041
6ug/mL	1.091	1.11	1.081
	1.129
3ug/mL	0.503	0.526	0.497
	0.549
1.5ug/mL	0.319	0.291	0.262
	0.263
0.75ug/mL	0.118	0.103	0.073
	0.087
0ug/mL	0.026	0.029	0
	0.032

Gene ID	25081
UniProt ID	P04639

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111999	E0743Ra	48T	$320		Add to Cart
BHE12111999	E0743Ra	96T	$458		Add to Cart

Rat Apolipoprotein A1 - APO-A1 ELISA Kit

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