Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Anti-KLH IgG and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-KLH IgG in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-100
94
EDTA plasma(n=5)
87-103
92
heparin plasma(n=5)
86-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-KLH IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-101%
89-100%
87-101%
EDTA plasma(n=5)
83-98%
89-101%
88-101%
heparin plasma(n=5)
80-95%
81-99%
82-97%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
Anti-KLH IgGELISA kit allows for the in vitro quantitative determination of Anti-KLH IgG concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat Anti-KLH IgG (Keyhole Limpet Hemocyanin IgG) ELISA kit