Rat Angiotensin converting enzyme 2, ACE2 ELISA Kit

Mfr.No.

CSB-E14308r

Sensitivity

0.02 ng/ml

Detection Range

0.02 ng/ml - 20 ng/ml

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Recovery

The recovery of rat PL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	96-106
EDTA plasma (n=4)	94	87-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat PL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	106
	Range %	103-109
1:2	Average %	96
	Range %	91-101
1:4	Average %	92
	Range %	87-97
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

UniProt ID	Q5EGZ1
Tissue Specificity	Expressed in heart, kidney and forebrain. In testis, expression is restricted to Leydig cells. In heart, expressed in endothelial cells from small and large arteries, arterial smooth muscle cells, and myocytes (at protein level). Ubiquitously expressed, w

Applications	ELISA
Research Area	ACE2

Storage	Store at 2-8°C. Please refer to protocol.

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